An Optical/Photoacoustic Dual-Modality Probe: Ratiometric in/ex Vivo Imaging for Stimulated H2S Upregulation in Mice

• Published in: Journal of the American Chemical Society Vol. 141; no. 45; pp. 17973 - 17977
• Main Authors: Chen, Zhongyan, Mu, Xueling, Han, Zhong, Yang, Shiping, Zhang, Changli, Guo, Zijian, Bai, Yang, He, Weijiang
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 13.11.2019; Amer Chemical Soc
• Subjects: Chemistry; Chemistry, Multidisciplinary; fluorescence; hydrogen sulfide; image analysis; intravenous injection; liver; mice; Physical Sciences; S-adenosylmethionine; Science & Technology; DYE; SIGNALING PATHWAYS; NANOPROBE; STRATEGY; HYDROGEN-SULFIDE; LEVEL; CANCER; FLUORESCENCE PROBES
• ISSN: 0002-7863; 1520-5126; 1520-5126
• DOI: 10.1021/jacs.9b09181

Tracking signaling H2S in live mice demands responsive imaging with fine tissue imaging depth and low interferences from tissue scattering/autofluorescence and probe concentration. With complementary advantages of fluorescence and photoacoustic (PA) imaging, optical/PA dual-modality imaging was suggested for in/ex vivo H2S imaging. Therefore, a meso-benzoyloxyltricarbo­heptamethine cyanine, HS-CyBz, was prepared as the first ratiometric optical/PA dual-modality probe for H2S, profiting from a keto–enol transition sensing mechanism. Tail intravenous injection of this probe leads to probe accumulation in the liver of mice, and the endogenous H2S upregulation triggered by S-adenosyl-l-methionine has been verified by ratiometric optical/PA imaging, suggesting the promising potential of this ratiometric dual-modality imaging.