Sequence-Specific Cu(II)-Dependent Peptide Bond Hydrolysis: Similarities and Differences with the Ni(II)-Dependent Reaction

• Published in: Inorganic chemistry Vol. 53; no. 9; pp. 4639 - 4646
• Main Authors: Belczyk-Ciesielska, Agnieszka, Zawisza, Izabela A, Mital, Mariusz, Bonna, Arkadiusz, Bal, Wojciech
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.05.2014
• Subjects: Chromatography, High Pressure Liquid; Copper - chemistry; Hydrolysis; Nickel - chemistry; Peptides - chemistry; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet

Potentiometry and UV–vis and circular dichroism spectroscopies were applied to characterize Cu(II) coordination to the Ac-GASRHWKFL-NH2 peptide. Using HPLC and ESI-MS, we demonstrated that Cu(II) ions cause selective hydrolysis of the Ala-Ser peptide bond in this peptide and characterized the pH and...