Actin Stimulates Reduction of the MICAL‑2 Monooxygenase Domain

• Published in: Biochemistry (Easton) Vol. 52; no. 35; pp. 6076 - 6084
• Main Authors: McDonald, Claudia A, Liu, Ying Yi, Palfey, Bruce A
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.09.2013
• Subjects: Actins - chemistry; Animals; Base Sequence; DNA Primers; Kinetics; Mice; Microtubule-Associated Proteins - chemistry; Mixed Function Oxygenases - chemistry; Models, Molecular; NAD - chemistry; Oxidation-Reduction; Stereoisomerism

MICALs are large, multidomain flavin-dependent monooxygenases that use redox chemistry to cause actin to depolymerize. Little enzymology has been reported for MICALs, and none has been reported for MICAL-2, an enzyme vital for the proliferation of prostate cancer. The monooxygenase domains of MICALs...