Hyperpolarized 13C Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood–Brain Barrier in the Cerebral Uptake and Metabolism of l‑Lactate in Vivo

• Published in: ACS Chemical Neuroscience Vol. 9; no. 11; pp. 2554 - 2562
• Main Authors: Takado, Yuhei, Cheng, Tian, Bastiaansen, Jessica A. M, Yoshihara, Hikari A. I, Lanz, Bernard, Mishkovsky, Mor, Lengacher, Sylvain, Comment, Arnaud
• Format: Journal Article
• Language: English; Japanese
• Published: American Chemical Society 17.05.2018; American Chemical Society (ACS)
• Subjects: Animals; Blood-Brain Barrier/metabolism; Blood-Brain Barrier/radiation effects; Brain/metabolism; Brain/radiation effects; Carbon-13 Magnetic Resonance Spectroscopy; L-Lactate Dehydrogenase/genetics; L-Lactate Dehydrogenase/metabolism; Lactic Acid/metabolism; Lactic Acid/radiation effects; Male; Mice; Mice, Inbred C57BL; Pyruvic Acid/metabolism; Pyruvic Acid/radiation effects; Ultrasonic Waves; Hyperpolarization; bicarbonate; dynamic nuclear polarization; magnetic resonance spectroscopy; pyruvate; ultrasound; bicarbonate; CIBM-AIT; dynamic nuclear polarization; exchange; hyperpolarization; infusion; inhibition; ldh; magnetic resonance spectroscopy; mrs; pyruvate; substrate; ultrasound; magnetic resonance spectroscopy; dynamic nuclear polarization; bicarbonate; pyruvate; Hyperpolarization; ultrasound

The dynamics of l-lactate transport across the blood–brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized 13C magnetic resonance spectroscopy (MRS). Following the intravenous inject...