Use of Peptide Microarrays for Fast and Informative Profiling of Therapeutic Antibody Formulation Conditions

• Published in: Molecular pharmaceutics Vol. 18; no. 11; pp. 4131 - 4139
• Main Authors: Austerberry, James, Edwards, John, Eyes, Tim, Derrick, Jeremy P
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.11.2021
• Subjects: Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - therapeutic use; Biological Products - chemistry; Biological Products - therapeutic use; Chemistry, Pharmaceutical - methods; Humans; Machine Learning; Peptides - chemistry; Protein Array Analysis - methods; Protein Stability; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - therapeutic use; formulation; peptide; microarray; biopharmaceutical; monoclonal antibody
• ISSN: 1543-8384; 1543-8392; 1543-8392
• DOI: 10.1021/acs.molpharmaceut.1c00543

Methods to optimize the solution behavior of therapeutic proteins are frequently time-consuming, provide limited information, and often use milligram quantities of material. Here, we present a simple, versatile method that provides valuable information to guide the identification and comparison of f...