Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis
In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary levels. The design is based on the immobilization o...
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Published in | Journal of chemical education Vol. 84; no. 4; pp. 677 - 680 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Easton
Division of Chemical Education
01.04.2007
Division of Chemical Education of the American Chemical Society American Chemical Society |
Subjects | |
Online Access | Get full text |
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Abstract | In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary levels. The design is based on the immobilization of the glucose oxidase and horseradish peroxidase enzymes with a redox mediator (ferrocene) in a carbon paste electrode. The glucose concentration is directly related to the cathodic current yielded by the oxidized form of the mediator obtained in the enzymatic reactions. The students use cyclic voltammetry to optimize sensor design and performance, and analytical characterization and glucose determination is carried out amperometrically. The preparation of the sensor is simple, quick, and can be carried out with inexpensive materials. The challenge of constructing biosensing devices and the good results typically obtained are very stimulating for the undergraduate students. |
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AbstractList | The construction of an amperometric biosensor for glucose analysis is described demonstrating that the analysis is easy to perform and the biosensor gives good analytical performance. This experiment helped the students to acquire problem-solving and teamwork skills, allowing them to reach a high level of independent and critical thought. In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary levels. The preparation of the sensor is simple, quick, and can be carried out with inexpensive materials. In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary levels. The design is based on the immobilization of the glucose oxidase and horseradish peroxidase enzymes with a redox mediator (ferrocene) in a carbon paste electrode. The glucose concentration is directly related to the cathodic current yielded by the oxidized form of the mediator obtained in the enzymatic reactions. The students use cyclic voltammetry to optimize sensor design and performance, and analytical characterization and glucose determination is carried out amperometrically. The preparation of the sensor is simple, quick, and can be carried out with inexpensive materials. The challenge of constructing biosensing devices and the good results typically obtained are very stimulating for the undergraduate students. |
Audience | Higher Education |
Author | Blanco-López, M. C Miranda-Ordieres, A. J Lobo-Castañón, M. J |
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Snippet | In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is... The construction of an amperometric biosensor for glucose analysis is described demonstrating that the analysis is easy to perform and the biosensor gives good... |
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SubjectTerms | Analytical chemistry Beverages Biosensors Chemistry College Science DIY Do it yourself Electrical measurement Glucose Laboratory Equipment Laboratory Experiments Measurement Equipment Measurement techniques Science education Science Equipment Science Experiments Science Instruction Scientific apparatus & instruments Soft drinks Teaching methods Undergraduate Study |
Title | Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis |
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