Detecting Ligand Binding to a Small RNA Target via Saturation Transfer Difference NMR Experiments in D2O and H2O

• Published in: Journal of the American Chemical Society Vol. 124; no. 45; pp. 13376 - 13377
• Main Authors: Mayer, Moriz, James, Thomas L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 13.11.2002
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Molecular genetics; Transcription. Transcription factor. Splicing. Rna processing; Saturation transfer; Binding site; NMR spectrometry; RNA; Ligand

A small RNA motif is used as a target for ligand-based NMR-screening by saturation transfer difference (STD) NMR experiments. The prerequisites for using a small RNA target in STD experiments, such as saturation time, frequency, and pulses, are discussed. We also show that it is of advantage to use...