Optimizing Transfection Efficiency in CAR‑T Cell Manufacturing through Multiple Administrations of Lipid-Based Nanoparticles

• Published in: ACS applied bio materials Vol. 7; no. 6; pp. 3746 - 3757
• Main Authors: Giulimondi, Francesca, Digiacomo, Luca, Renzi, Serena, Cassone, Chiara, Pirrottina, Andrea, Molfetta, Rosa, Palamà, Ilaria Elena, Maiorano, Gabriele, Gigli, Giuseppe, Amenitsch, Heinz, Pozzi, Daniela, Zingoni, Alessandra, Caracciolo, Giulio
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.05.2024
• Subjects: gene delivery; cell transfection; lipid nanoparticles; CAR-T; lipoplexes

The existing manufacturing protocols for CAR-T cell therapies pose notable challenges, particularly in attaining a transient transfection that endures for a significant duration. To address this gap, this study aims to formulate a transfection protocol utilizing multiple lipid-based nanoparticles (L...