Dendritic cell identification in head and neck lymphoid tissue. Newly recognized cells control T-lymphocyte functions
The physiologic measurements of a subpopulation of mononuclear cells derived from head and neck lymphoid tissues are similar to those of dendritic cells are described. Dendritic cells are a subpopulation of bone marrow-derived leukocytes that were originally identified in rodents and now described i...
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Published in | Archives of otolaryngology (1960) Vol. 110; no. 11; p. 701 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.11.1984
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Subjects | |
Online Access | Get more information |
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Summary: | The physiologic measurements of a subpopulation of mononuclear cells derived from head and neck lymphoid tissues are similar to those of dendritic cells are described. Dendritic cells are a subpopulation of bone marrow-derived leukocytes that were originally identified in rodents and now described in man as having central control of T-lymphocyte functions. We describe a technique for the enrichment of dendritic cells obtained from tonsils utilizing a bovine serum albumin (BSA) gradient and note that they have the light and electron microscopic appearance of dendritic cells. The measured oxidative mitogenic response and interferon-gamma production in complete leukocyte cultures was compared with BSA gradient-separated preparations. The denser cells, comprised mostly of normal appearing lymphocytes, would not undergo a mitogenic response nor produce normal amounts of interferon when stimulated unless the dendritic cell-rich, less-dense fraction, was added back. The dendritic cells derived from tonsils seem to behave as a potent accessory cell for these T-lymphocyte-associated functions. |
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ISSN: | 0003-9977 |
DOI: | 10.1001/archotol.1984.00800370003001 |