Determination of Some Physicochemical Properties of Mebendazole with RPLC Method

• Published in: Journal of chemical and engineering data Vol. 64; no. 6; pp. 2736 - 2741
• Main Authors: Poturcu, Kader, Çubuk Demiralay, Ebru
• Format: Journal Article
• Language: English
• Published: American Chemical Society 13.06.2019
• ISSN: 0021-9568; 1520-5134
• DOI: 10.1021/acs.jced.9b00131

In this study, the retention behavior of benzimidazole anthelmintic drug mebendazole (MBZ) was investigated with a liquid chromatographic method. Influence of acetonitrile (ACN) concentration present in the mobile phase on retention measurement was examined. Analyses were carried out with a Kinetex Core–Shell C8 (Phenomenex, 150 × 4.6 mm I.D., 2.6 μm) column at 25 °C. The mobile phase pH (s spH) values were measured in ACN + water mixtures. By using the retention data obtained from this chromatographic analysis, thermodynamic dissociation constant (pK a) values of the mebendazole were investigated experimentally at a constant flow rate. Experimental data were analyzed by using a nonlinear regression program. This is the first time that the pH–retention time relationships of MBZ were evaluated by the experimental method. Moreover, the pK a values at 37 °C, solubility (log S w), and lipophilicity (log P o/w) were calculated by using Abraham’s solute descriptors. The pK a values obtained from this study complied with the literature data acquired from other methods.