Solid-State NMR of a Protein in a Precipitated Complex with a Full-Length Antibody

• Published in: Journal of the American Chemical Society Vol. 136; no. 48; pp. 16800 - 16806
• Main Authors: Lamley, Jonathan M, Iuga, Dinu, Öster, Carl, Sass, Hans-Juergen, Rogowski, Marco, Oss, Andres, Past, Jaan, Reinhold, Andres, Grzesiek, Stephan, Samoson, Ago, Lewandowski, Józef R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.12.2014
• Subjects: Antigen-Antibody Complex - chemistry; Antigen-Antibody Complex - immunology; Chemical Precipitation; Humans; Immunoglobulins - chemistry; Immunoglobulins - immunology; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Proteins - chemistry; Proteins - immunology

NMR spectroscopy is a prime technique for characterizing atomic-resolution structures and dynamics of biomolecular complexes but for such systems faces challenges of sensitivity and spectral resolution. We demonstrate that the application of 1H-detected experiments at magic-angle spinning frequencie...