An Approach towards the Measurement of Nanometer Range Distances Based on Cu2+ Ions and ESR

• Published in: The journal of physical chemistry. B Vol. 114; no. 18; pp. 6165 - 6174
• Main Authors: Yang, Zhongyu, Kise, Drew, Saxena, Sunil
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13.05.2010
• Subjects: B: Biophysical Chemistry

We present the measurement of Cu2+−Cu2+ and Cu2+−nitroxide distance distributions using double electron−electron resonance (DEER) on a proline-based peptide and an alanine-based peptide. The proline-based peptide contains two well-characterized Cu2+ binding segments, PHGGGW, separated by seven proli...