Formation of Gel-like Nanodomains in Cholesterol-Containing Sphingomyelin or Phosphatidylcholine Binary Membrane As Examined by Fluorescence Lifetimes and 2H NMR Spectra

• Published in: Langmuir Vol. 31; no. 51; pp. 13783 - 13792
• Main Authors: Yasuda, Tomokazu, Matsumori, Nobuaki, Tsuchikawa, Hiroshi, Lönnfors, Max, Nyholm, Thomas K. M., Slotte, J. Peter, Murata, Michio
• Format: Journal Article
• Language: English
• Published: American Chemical Society 29.12.2015
• Subjects: cholesterol; deuterium; fluorescence; nuclear magnetic resonance spectroscopy; phosphatidylcholines; sphingomyelins; stable isotopes; temperature; thermal stability

In this study, we measured the time-resolved fluorescence of trans-parinaric acid (tPA), steady-state fluorescence anisotropy of diphenylhexatriene (DPH), and 2H NMR of 10,10-d 2-stearoyl lipids in stearoyl sphingomyelin with cholesterol (SSM/Chol) and l-palmitoyl-2-stearoyl-sn-glycero-3-phosphochol...