Bound Peptide-Dependent Thermal Stability of Major Histocompatibility Complex Class II Molecule I-Ek

• Published in: Biochemistry (Easton) Vol. 43; no. 31; pp. 10186 - 10191
• Main Authors: Saito, Keigo, Oda, Masayuki, Sarai, Akinori, Azuma, Takachika, Kozono, Haruo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 10.08.2004
• Subjects: Animals; Antigen Presentation; Aspartic Acid - genetics; Calorimetry, Differential Scanning; CD4-Positive T-Lymphocytes - metabolism; Chromatography, Gel; Glutamic Acid - genetics; Hemoglobins - chemistry; Hemoglobins - genetics; Hemoglobins - metabolism; Histocompatibility Antigens Class II - chemistry; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - metabolism; Hydrogen-Ion Concentration; Mice; Mutagenesis, Site-Directed; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Protein Binding; Protein Conformation; Protein Denaturation; Thermodynamics

We used differential scanning calorimetry to study the thermal denaturation of murine major histocompatibility complex class II, I-Ek, accommodating hemoglobin (Hb) peptide mutants possessing a single amino acid substitution of the chemically conserved amino acids buried in the I-Ek pocket (position...