Chlorophyll Fluorescence Understanding Crop Performance–Basics and Applications

Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Under...

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Main Authors Kalaji, Mohamed H., Goltsev, Vasilij N., Żuk-Golaszewska, Krystyna, Zivcak, Marek, Brestic, Marian
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LanguageEnglish
Published Boca Raton CRC Press 2017
Taylor & Francis Group
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Abstract Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Understanding Crop Performance — Basics and Applications reviews a diversity of instruments available for recording and analyzing different types of light signals from plants and addresses the use of chlorophyll a fluorescence in research on plants and other photosynthesizing organisms, such as algae and cyanobacteria. This book characterizes the phenomenon of chlorophyll a fluorescence, describes the methods for its measurement, and demonstrates — using selected examples — the applicability of these methods to research the response of the photosynthetic apparatus and plant tolerance to unfavorable environmental conditions. In addition, chapters cover a general background on photosynthesis, analysis of delayed fluorescence, and the pulse amplitude modulated (PAM) technique. The book is addressed to a wide range of professionals in photosynthesis research and scientists from other areas of plant sciences. Photosynthesis Fluorescence of chlorophyll a JIP (OJIP) test Delayed fluorescence in photosynthesis Pulse-amplitude modulated fluorescence (PAM) measurements Application of chlorophyll a fluorescence in plant research Hazem M. Kalaji is a Polish plant physiologist of Syrian origin. He has more than 30 years of experiences in the field of photosynthesis and stress physiology. After finishing his study at The Faculty of Agriculture - Aleppo University (Syria), he got his PhD degree from Agriculture Faculty (recently, Faculty of Agriculture and Biology) at Warsaw Agricultural University-SGGW (recently, Warsaw University of Life Sciences-SGGW). Recently, he is working as Associate Professor at the same university as a lecturer (Plant physiology; Yielding biology and biological progress; The use of computerized equipment in plant physiological studies and agricultural sciences; Physiology of crop yield; Biological progress and yield biology; Physiology of plants and its production under climate change; Photosynthetic crop productivity under unfavourable environmental conditions; Energy crops: a renewable energy source; Climate change impacts on plant growth and crop yield) and researcher (photosynthesis, chlorophyll fluorescence; abiotic and biotic stress; nutrient deficiency; climate change; crops production). Hazem M. Kalaji has been working as international advisor and cooperates with more than 50 well known international institutes, laboratories and companies that develop instrumentations to be used in the field of plant physiology. He is working as Vice Cahir of MSCA at the Scientific Committee of European Union. He already published 4 books (in Polish, Italian and Russian) and more than 100 scientific papers. Besides, he is working as Associate Editor of Photosynthetica Journal and belongs to The Editorial Board of 7 international scientific journals. He organized many world-wide conferences and participated in more than 150 meetings, symposiums and seminars. Vasilij Goltsev is a Professor at St. Kliment Ohridski University of Sofia, Bulgaria. For 18 years he was The Head of the Department of Biophysics and Radiobiology, Faculty of Biology at University of Sofia. Prof. Goltsev dedicated more than 35 years towards research of light emission from plants and teaching biophysics, photobiology, biophysics of photosynthesis, mathematical modeling. His publications are in field of plant stress response and adaptation to adverse environmental, physical, chemical and biological factors. Along with his team he has been working on biophysical aspects of stress response of photosynthetic machinery and on development of a luminescent approach for multi-parametric estimation of the physiological state and stress reaction in plants at in vivo and in situ condition. V. Goltsev currently is in a member of Editorial Board of the following journals: Genetics and Plant Physiology, NanoPhotoBioSciences, Technology of Living Systems, Review Editor of the journal Agroecology and Land Use Systems. Krystyna Żuk-Gołaszewska (Ph.D. in agronomy) is the Associate Professor with 24-year research and didactic experience at the University of Warmia and Mazury in Olsztyn, the Department of Agrotechnology, Agricultural Production Management and Agribusiness, Poland. Her scientific activities are focused on tolerance to biotic and abiotic stresses of agricultural crops (e.g. Lupinus luteus, Vicia faba, Pisum sativum, Trifolium pretense, Triticum spp., Hordeum vulgare) and medical plants (e.g. Trigonella foenum-graecum, Nigella sativa); organic farming system with ancient wheats; precision agriculture (legumes); and agricultural methods of biofortification (wheats). The integral part of her research is to determine the relation between agrotechnical factors of crop production and physiological parameters of plant growth and development associated with measurements and indices of photosynthetic efficiency, transpiration, water use efficiency, LAI, chlorophyll content, and others. She co-authored two chapters of the book "Organic Crop Husbandry"; serves as a member of Editorial Board of the Global Journal of Botanical Sciences and the Journal of Agri-Food and Applied Sciences; reviews scientific papers. The international research experience and skills has been gained in Canada (University of British Columbia, Vancouver – visiting professor), the Netherlands (Plant Research International, Wageningen – scholarship), and Israel (Volcani Center, Bet Dagan – traineeship). Her didactic works on agronomy subjects at the mother University have been accompanied with an international activity as visiting professor in Italian (University of Bologna, Faculty Science of Agriculture) and Armenian (Agrarian University in Yerevan) universities.
AbstractList Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Understanding Crop Performance -- Basics and Applications reviews a diversity of instruments available for recording and analyzing different types of light signals from plants and addresses the use of chlorophyll a fluorescence in research on plants and other photosynthesizing organisms, such as algae and cyanobacteria.
Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Understanding Crop Performance - Basics and Applications reviews a diversity of instruments available for recording and analyzing different types of light signals from plants and addresses the use of chlorophyll a fluorescence in research on plants and other photosynthesizing organisms, such as algae and cyanobacteria. This book characterizes the phenomenon of chlorophyll a fluorescence, describes the methods for its measurement, and demonstrates - using selected examples - the applicability of these methods to research the response of the photosynthetic apparatus and plant tolerance to unfavorable environmental conditions. In addition, chapters cover a general background on photosynthesis, analysis of delayed fluorescence, and the pulse amplitude modulated (PAM) technique. The book is addressed to a wide range of professionals in photosynthesis research and scientists from other areas of plant sciences.
Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor stresses including mineral deficiencies, soil salinity, and pathogenic diseases. This book reviews a diversity of instruments available for recording and analysing all types of light signals from plants.
Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Understanding Crop Performance — Basics and Applications reviews a diversity of instruments available for recording and analyzing different types of light signals from plants and addresses the use of chlorophyll a fluorescence in research on plants and other photosynthesizing organisms, such as algae and cyanobacteria. This book characterizes the phenomenon of chlorophyll a fluorescence, describes the methods for its measurement, and demonstrates — using selected examples — the applicability of these methods to research the response of the photosynthetic apparatus and plant tolerance to unfavorable environmental conditions. In addition, chapters cover a general background on photosynthesis, analysis of delayed fluorescence, and the pulse amplitude modulated (PAM) technique. The book is addressed to a wide range of professionals in photosynthesis research and scientists from other areas of plant sciences. Photosynthesis Fluorescence of chlorophyll a JIP (OJIP) test Delayed fluorescence in photosynthesis Pulse-amplitude modulated fluorescence (PAM) measurements Application of chlorophyll a fluorescence in plant research Hazem M. Kalaji is a Polish plant physiologist of Syrian origin. He has more than 30 years of experiences in the field of photosynthesis and stress physiology. After finishing his study at The Faculty of Agriculture - Aleppo University (Syria), he got his PhD degree from Agriculture Faculty (recently, Faculty of Agriculture and Biology) at Warsaw Agricultural University-SGGW (recently, Warsaw University of Life Sciences-SGGW). Recently, he is working as Associate Professor at the same university as a lecturer (Plant physiology; Yielding biology and biological progress; The use of computerized equipment in plant physiological studies and agricultural sciences; Physiology of crop yield; Biological progress and yield biology; Physiology of plants and its production under climate change; Photosynthetic crop productivity under unfavourable environmental conditions; Energy crops: a renewable energy source; Climate change impacts on plant growth and crop yield) and researcher (photosynthesis, chlorophyll fluorescence; abiotic and biotic stress; nutrient deficiency; climate change; crops production). Hazem M. Kalaji has been working as international advisor and cooperates with more than 50 well known international institutes, laboratories and companies that develop instrumentations to be used in the field of plant physiology. He is working as Vice Cahir of MSCA at the Scientific Committee of European Union. He already published 4 books (in Polish, Italian and Russian) and more than 100 scientific papers. Besides, he is working as Associate Editor of Photosynthetica Journal and belongs to The Editorial Board of 7 international scientific journals. He organized many world-wide conferences and participated in more than 150 meetings, symposiums and seminars. Vasilij Goltsev is a Professor at St. Kliment Ohridski University of Sofia, Bulgaria. For 18 years he was The Head of the Department of Biophysics and Radiobiology, Faculty of Biology at University of Sofia. Prof. Goltsev dedicated more than 35 years towards research of light emission from plants and teaching biophysics, photobiology, biophysics of photosynthesis, mathematical modeling. His publications are in field of plant stress response and adaptation to adverse environmental, physical, chemical and biological factors. Along with his team he has been working on biophysical aspects of stress response of photosynthetic machinery and on development of a luminescent approach for multi-parametric estimation of the physiological state and stress reaction in plants at in vivo and in situ condition. V. Goltsev currently is in a member of Editorial Board of the following journals: Genetics and Plant Physiology, NanoPhotoBioSciences, Technology of Living Systems, Review Editor of the journal Agroecology and Land Use Systems. Krystyna Żuk-Gołaszewska (Ph.D. in agronomy) is the Associate Professor with 24-year research and didactic experience at the University of Warmia and Mazury in Olsztyn, the Department of Agrotechnology, Agricultural Production Management and Agribusiness, Poland. Her scientific activities are focused on tolerance to biotic and abiotic stresses of agricultural crops (e.g. Lupinus luteus, Vicia faba, Pisum sativum, Trifolium pretense, Triticum spp., Hordeum vulgare) and medical plants (e.g. Trigonella foenum-graecum, Nigella sativa); organic farming system with ancient wheats; precision agriculture (legumes); and agricultural methods of biofortification (wheats). The integral part of her research is to determine the relation between agrotechnical factors of crop production and physiological parameters of plant growth and development associated with measurements and indices of photosynthetic efficiency, transpiration, water use efficiency, LAI, chlorophyll content, and others. She co-authored two chapters of the book "Organic Crop Husbandry"; serves as a member of Editorial Board of the Global Journal of Botanical Sciences and the Journal of Agri-Food and Applied Sciences; reviews scientific papers. The international research experience and skills has been gained in Canada (University of British Columbia, Vancouver – visiting professor), the Netherlands (Plant Research International, Wageningen – scholarship), and Israel (Volcani Center, Bet Dagan – traineeship). Her didactic works on agronomy subjects at the mother University have been accompanied with an international activity as visiting professor in Italian (University of Bologna, Faculty Science of Agriculture) and Armenian (Agrarian University in Yerevan) universities.
Author Goltsev, Vasilij N.
Zivcak, Marek
Żuk-Golaszewska, Krystyna
Kalaji, Mohamed H.
Brestic, Marian
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Keywords Chlorophyll Fluorescence Imaging
Chlorophyll Fluorescence Induction
Chlorophyll Fluorescence Parameters
PSII Quantum Yield
Chlorophyll Fluorescence Measurements
Induction Curve
Quantum Yield
PSII RCs
NPQ
Fluorescence Parameters
Fluorescence Induction Curve
Calvin Benson Cycle
Chloroplast Movement
Chlorophyll Fluorescence
Electron Transport Rate
PSII Photochemistry
Psi RC
Rapid Light Curves
JIP Test
Thylakoid Membranes
Pq Pool
Actinic Light
Hansatech Instruments
Open PSII RCs
Protein D1
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Notes Includes bibliographical references and index
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Snippet Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs...
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proquest
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SourceType Aggregation Database
Publisher
SubjectTerms AGRICULTUREnetBASE
Biodiversity
BIOSCIENCEnetBASE
Botany
Crop Science
ENVIROnetBASE
ENVIRONMENTALSCIENCEnetBASE
heavy metals stress, drought, flooding
LIFESCIENCEnetBASE
light
mineral deficiencies, soil salinity, and pathogenic diseases; plant stress
Photosynthesis
Photosynthesis -- Measurement
photosynthesis, photosystem I & II, UV light
plant phenotyping; plant breeding; imaging techniques
Plants
Plants - Effect of stress on
PlantSCIENCEnetBASE
salinity
SCI-TECHnetBASE
STMnetBASE
temperature
water
Subtitle Performance–Basics and Applications
TableOfContents Cover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Acknowledgments -- Authors -- Introduction -- 1: Photosynthesis -- 1.1 General Description of Photosynthesis -- 1.1.1 PSII Antenna Complex -- 1.1.2 Photosynthetic Pigments of Higher Plants -- 1.1.3 Photosystem II (PSII) -- 1.1.4 Cytochrome  b6 f Complex -- 1.1.5 Photosystem I (PSI) -- 1.1.6 ATP Synthase -- 1.2 Light Phase of Photosynthesis -- 1.2.1 Absorption of Photosynthetically Active Radiation (PAR) -- 1.2.2 Transport of Electrons and Non-Cyclic Phosphorylation -- 1.2.3 Cyclic Transport of Electrons and Cyclic Photophosphorylation -- 1.2.4 Pseudocyclic Transport of Electrons and Pseudocyclic Photophosphorylation -- References -- 2: Fluorescence of Chlorophyll  a -- 2.1 Chlorophyll a  Fluorescence as an Indicator of PSII Performance -- 2.2 Chlorophyll a  Fluorescence Induction Curve -- 2.3 Techniques for Measuring Chlorophyll a  Fluorescence -- 2.3.1 Direct Fluorescence of Chlorophyll  a -- 2.3.2 Modulated Chlorophyll  a Fluorescence -- References -- 3: JIP (OJIP) Test -- 3.1 Parameters in JIP (OJIP) Test -- 3.2 Data Derived Indirectly from JIP Test (Strasser et al. 2000) -- 3.3 Fluorescence Parameters Derived from Measured Data -- 3.4 Specific Energy Fluxes per QA -Reduced PSII Reaction Center -- 3.5 Yields or Energy Flux Ratios -- 3.6  PHENOMENOLOGICAL ENERGY FLUXES PER EXCITED CROSS-SECTION (CS) OF A SAMPLE -- 3.7 PERFORMANCE INDICES AT t   =  0 AND DENSITY OF ACTIVE PSII REACTION CENTERS -- 3.8 DRIVING FORCES (LOGARITHMS OF PERFORMANCE INDICES AT t   =  0) -- 3.9 OVERALL GROUPING PROBABILITY -- 3.10 STRUCTURE AND FUNCTION INDICES -- 3.11 ASSOCIATION OF OJIP FLUORESCENCE KINETICS WITH MEASUREMENTS OF LIGHT SCATTERING AT 820  NM WAVELENGTH -- REFERENCES -- 4: Delayed Fluorescence in Photosynthesis -- 4.1 Basics of Delayed Light Emission by Plants
5.3.2.1.2 Physiological Meaning and Application of the Parameter -- 5.3.2.1. 3 Determination of the Parameter and the Related Pitfalls -- 5.3.2.2 Non-Photochemical Quenching of Variable Fluorescence, qN -- 5.3.2.2.1 Theoretical Definition and Determination -- 5.3.2.2.2 Physiological Meaning and Application of the Parameter -- 5.3.2.3 Non-Photochemical Quenching of Maximum Fluorescence, NPQ -- 5.3.2.3.1 Theoretical Definition -- 5.3.2.3.2 Physiological Meaning and Application of the Parameter -- 5.3.2.3.3 Determination of the Parameter and the Related Pitfalls -- 5.3.2.4 Fractions of Non-Photochemical Quenching -- 5.3.3 Parameters of Energy Partitioning -- 5.3.3.1 Rationale of Energy Partitioning -- 5.3.3.2 Maximum Quantum Yield of PSII Photochemistry, Φ Po, FV /FM -- 5.3.3.2.1 Theoretical Definition -- 5.3.3.2.2 Physiological Meaning and Application of the Parameter -- 5.3.3.2.3 Determination of the Parameter and the Related Pitfalls -- 5.3.3.3 Maximum Quantum Yield of Basal Non-Photochemical Energy Losses, Φ No, F0 /FM -- 5.3.3.3.1 Theoretical Definition and Determination -- 5.3.3.3.2 Physiological Meaning and Application of the Parameter -- 5.3.3.3.3 Determination of the Parameter and the Related Pitfalls -- 5.3.3.4 Maximum PSII Quantum Yield in a Light-Adapted Sample, Φ P , FV ʹ /FM ʹ -- 5.3.3.4.1 Theoretical Definition and Calculation of the Parameter -- 5.3.3.4.2 Physiological Meaning and Application of the Parameter -- 5.3.3.5 Efficient Quantum Yield at a Given Light Intensity -- 5.3.3.5.1 Theoretical Definition and Calculation -- 5.3.3.5.2 Physiological Meaning and Application -- 5.3.3.6 Quantum Yield of Non-Light-Induced Energy Losses -- 5.3.3.6.1 Theoretical Definition and Calculation -- 5.3.3.6.2 Physiological Meaning and Application -- 5.3.3.7 Quantum Yield of Light-Induced Energy Losses -- 5.3.3.7.1 Theoretical Definition and Calculation
4.1.1 Mechanism of DF Quanta Generation -- 4.1.2 Decay Kinetics of Delayed Fluorescence -- 4.1.3 Induction Kinetics of Delayed Fluorescence -- 4.2 Analysis of Relationship Between Delayed and Variable ChlorophylL a  Fluorescence -- 4.3 Methods for Recording Delayed Fluorescence -- References -- 5: Pulse-Amplitude Modulated (PAM) Fluorescence Measurements -- 5.1 Rationale for the Saturation Pulse Method -- 5.2 Interpretation of the Saturation Pulse Method with the Use of Mathematical Models -- 5.2.1 Processes Affecting Fluorescence Emission in Terms of Their Rate Constants -- 5.2.2 Sensitivity Factor  S -- 5.2.3 PSI Fluorescence -- 5.3 Parameters Derived from Pulse-Amplitude Measurements -- 5.3.1 Directly Measured Basic Parameters -- 5.3.1.1 Basal Fluorescence F0 -- 5.3.1.1.1 Theoretical Definition -- 5.3.1.1.2 Physiological Meaning and Application of the Parameter -- 5.3.1.2 Maximum Fluorescence FM -- 5.3.1.2.1 Theoretical Definition -- 5.3.1.2.2 Physiological Meaning and Application of the Parameter -- 5.3.1.3 Variable Fluorescence FV -- 5.3.1.3.1 Theoretical Definition -- 5.3.1.3.2 Physiological Meaning and Application of the Parameter -- 5.3.1.4 Steady-State Fluorescence Fs ʹ -- 5.3.1.4.1 Theoretical Definition -- 5.3.1.4.2 Physiological Meaning and Application of the Parameter -- 5.3.1.5 Minimum Fluorescence in Light-Exposed Samples, F0 ʹ -- 5.3.1.5.1 Theoretical Definition -- 5.3.1.5.2 Physiological Meaning and Application of the Parameter -- 5.3.1.5.3 Determination of the Parameter and the Related Pitfalls -- 5.3.1.6 Maximum Fluorescence in Light-Exposed Samples, FM ʹ -- 5.3.1.6.1 Theoretical Definition -- 5.3.1.6.2 Physiological Meaning and Application of the Parameter -- 5.3.2 Parameters of Quenching Analysis -- 5.3.2.1 Photochemical Quenching of Variable Fluorescence qP, qL -- 5.3.2.1.1 Theoretical Definition
5.3.3.7.2 Physiological Meaning and Application -- 5.3.4 Electron Transport Rate (ETR PSII) -- 5.3.4.  1 Theoretical Definition and Calculation -- 5.3.4.2 Physiological Meaning and Application -- 5.3.4.  3 Limitations and Possible Errors in Estimations of Electron Transport Rate -- 5.3.4.3.1 Intensity of PAR Incident to the Leaf -- 5.3.4.3.2 Efficient Quantum Yield of PSII Photochemistry (Φ PSII ) -- 5.3.4.3.3 Leaf Absorbance (A) -- 5.3.4.3.4 Distribution of Absorbed Light between Two Photosystems -- 5.3.5 Summary of the Parameters -- 5.4 Most Useful Measurement Protocols Based on PAM Method -- 5.4.1 Single Saturation Pulse Applied to a Dark-Adapted Sample (F V/F M Measurement) -- 5.4.2 Simple Dark-to-Light Slow Fluorescence Induction -- 5.4.3 Measurements of Samples Not Adapted to Dark ( Ф PSII  Measurement) -- 5.4.4 Slow Induction Curve and Recovery -- 5.4.5 Light Response Curves -- 5.4.6 Relative Fluorescence Decrease -- 5.5 SPECIAL APPLICATIONS OF PAM METHOD -- 5.5.1 Chlorophyll Fluorescence Imaging -- 5.5.2 On-Site Long-Term Monitoring of Fluorescence Parameters -- 5.5.3 Measurement of Fluorescence Parameters under Multicolor Excitations -- 5.5.4 Simultaneous Measurements of PAM and Other Signals -- 5.5.4.1 Chlorophyll Fluorescence Measured Simultaneously with Photosynthetic Gas Exchange -- 5.5.4.2 Simultaneous Measurements of PSI Transmittance and Chlorophyll Fluorescence -- 5.5.5 Risks Associated with Multiple Saturation Pulse Methods -- REFERENCES -- 6: Application of Chlorophyll  a   Fluorescence in Plant Research -- 6.1 LIGHT STRESS -- 6.2 TEMPERATURE STRESS -- 6.3 WATER STRESS -- 6.3.1 Drought -- 6.3.2 Flooding Stress -- 6.4 SALINITY STRESS -- 6.5 STRESS CAUSED BY HEAVY METALS -- 6.6 NUTRIENT DEFICIENCY -- REFERENCES -- Index
Title Chlorophyll Fluorescence Understanding Crop
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