Cysteine metabolic engineering and selective disulfide reduction produce superior antibody-drug-conjugates
Procopio-Melino, Renée, Kotch, Frank W, Prashad, Amar S, Gomes, Jose M, Wang, Wenge, Arve, Bo, Dawdy, Andrew, Chen, Lawrence, Sperry, Justin, Hosselet, Christine, He, Tao, Kriz, Ronald, Lin, Laura, Marquette, Kimberly, Tchistiakova, Lioudmila, Somers, Will, Rouse, Jason C, Zhong, Xiaotian
Published in Scientific reports (04.05.2022)
Published in Scientific reports (04.05.2022)
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Journal Article
Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteins
Zhong, Xiaotian, Ma, Weijun, Meade, Caryl L., Tam, Amy S., Llewellyn, Eliza, Cornell, Richard, Cote, Kaffa, Scarcelli, John J., Marshall, Jeffrey K., Tzvetkova, Boriana, Figueroa, Bruno, DiNino, Dana, Sievers, Annette, Lee, Christopher, Guo, Jane, Mahan, Evan, Francis, Christopher, Lam, Khetemenee, D'Antona, Aaron M., Zollner, Richard, Zhu, Hongli L., Kriz, Ron, Somers, Will, Lin, Laura
Published in Biotechnology progress (01.01.2019)
Published in Biotechnology progress (01.01.2019)
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Journal Article
Pyroglutamate and O-Linked Glycan Determine Functional Production of Anti-IL17A and Anti-IL22 Peptide-Antibody Bispecific Genetic Fusions
Zhong, Xiaotian, Kieras, Elizabeth, Sousa, Eric, D'Antona, Aaron, Baber, J. Christian, He, Tao, Desharnais, Joel, Wood, Lauren, Luxenberg, Deborah, Stahl, Mark, Kriz, Ronald, Lin, Laura, Somers, Will, Fitz, Lori J., Wright, Jill F.
Published in The Journal of biological chemistry (11.01.2013)
Published in The Journal of biological chemistry (11.01.2013)
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Journal Article
Two N-Terminal Domains of Kv4 K + Channels Regulate Binding to and Modulation by KChIP1
Scannevin, Robert H, Wang, KeWei, Jow, Flora, Megules, Jennifer, Kopsco, David C, Edris, Wade, Carroll, Karen C, Lü, Qiang, Xu, Weixin, Xu, Zhangbao, Katz, Alan H, Olland, Stephane, Lin, Laura, Taylor, Meggin, Stahl, Mark, Malakian, Karl, Somers, Will, Mosyak, Lydia, Bowlby, Mark R, Chanda, Pranab, Rhodes, Kenneth J
Published in Neuron (Cambridge, Mass.) (19.02.2004)
Published in Neuron (Cambridge, Mass.) (19.02.2004)
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Journal Article
Improved variants of SrtA for site-specific conjugation on antibodies and proteins with high efficiency
Chen, Long, Cohen, Justin, Song, Xiaoda, Zhao, Aishan, Ye, Zi, Feulner, Christine J, Doonan, Patrick, Somers, Will, Lin, Laura, Chen, Peng R
Published in Scientific reports (18.08.2016)
Published in Scientific reports (18.08.2016)
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Journal Article
Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection
Zhong, Xiaotian, Schwab, Ashley, Ma, Weijun, Meade, Caryl L, Zhou, Jing, D'Antona, Aaron M, Somers, Will, Lin, Laura
Published in Methods in molecular biology (Clifton, N.J.) (2022)
Published in Methods in molecular biology (Clifton, N.J.) (2022)
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Journal Article
Glyco-engineered Long Acting FGF21 Variant with Optimal Pharmaceutical and Pharmacokinetic Properties to Enable Weekly to Twice Monthly Subcutaneous Dosing
Weng, Yan, Ishino, Tetsuya, Sievers, Annette, Talukdar, Saswata, Chabot, Jeffrey R, Tam, Amy, Duan, Weili, Kerns, Kelvin, Sousa, Eric, He, Tao, Logan, Alison, Lee, Darwin, Li, Dongmei, Zhou, Yingjiang, Bernardo, Barbara, Joyce, Alison, Kavosi, Mania, O'Hara, Denise M, Clark, Tracey, Guo, Jie, Giragossian, Craig, Stahl, Mark, Calle, Roberto A, Kriz, Ron, Somers, Will, Lin, Laura
Published in Scientific reports (09.03.2018)
Published in Scientific reports (09.03.2018)
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Journal Article
A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv)
Tu, Chao, Terraube, Virginie, Tam, Amy Sze Pui, Stochaj, Wayne, Fennell, Brian J., Lin, Laura, Stahl, Mark, LaVallie, Edward R., Somers, Will, Finlay, William J.J., Mosyak, Lydia, Bard, Joel, Cunningham, Orla
Published in The Journal of biological chemistry (15.01.2016)
Published in The Journal of biological chemistry (15.01.2016)
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Journal Article
Mechanistic understanding of the cysteine capping modifications of antibodies enables selective chemical engineering in live mammalian cells
Zhong, Xiaotian, He, Tao, Prashad, Amar S., Wang, Wenge, Cohen, Justin, Ferguson, Darren, Tam, Amy S., Sousa, Eric, Lin, Laura, Tchistiakova, Lioudmila, Gatto, Scott, D’Antona, Aaron, Luan, Yen-Tung, Ma, Weijun, Zollner, Richard, Zhou, Jing, Arve, Bo, Somers, Will, Kriz, Ronald
Published in Journal of biotechnology (20.04.2017)
Published in Journal of biotechnology (20.04.2017)
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Journal Article
Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: Molecular insights and effector modulation of N-acetylglucosaminyltransferase I
Zhong, Xiaotian, Cooley, Cecilia, Seth, Nilufer, Juo, Z. Sean, Presman, Ella, Resendes, Nicole, Kumar, Ravi, Allen, Martin, Mosyak, Lidia, Stahl, Mark, Somers, Will, Kriz, Ronald
Published in Biotechnology and bioengineering (01.07.2012)
Published in Biotechnology and bioengineering (01.07.2012)
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Journal Article
CAPPED AND UNCAPPED ANTIBODY CYSTEINES AND THEIR USE IN ANTIBODY-DRUG CONJUGATION
PRASHAD AMARNAUTH SHASTRIE, LETENDRE LEO JOSEPH, HE TAO, SOMERS WILL, WANG WENGE, ZHONG XIAOTIAN, KRIZ RONALD WILLIAM
Year of Publication 26.08.2020
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Year of Publication 26.08.2020
Patent
Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: Molecular insights and effector modulation of N-acetylglucosaminyltransferase I
Zhong, Xiaotian, Cooley, Cecilia, Seth, Nilufer, Juo, Z Sean, Presman, Ella, Resendes, Nicole, Kumar, Ravi, Allen, Martin, Mosyak, Lidia, Stahl, Mark, Somers, Will, Kriz, Ronald
Published in Biotechnology and bioengineering (01.07.2012)
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Published in Biotechnology and bioengineering (01.07.2012)
Journal Article
CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery
Fennell, Brian J, McDonnell, Barry, Tam, Amy Sze Pui, Chang, Lijun, Steven, John, Broadbent, Ian D, Gao, Huilan, Kieras, Elizabeth, Alley, Jennifer, Luxenberg, Deborah, Edmonds, Jason, Fitz, Lori J, Miao, Wenyan, Whitters, Matthew J, Medley, Quintus G, Guo, Yongjing J, Darmanin-Sheehan, Alfredo, Autin, Bénédicte, Shúilleabháin, Deirdre Ní, Cummins, Emma, King, Amy, Krebs, Mark RH, Grace, Christopher, Hickling, Timothy P, Boisvert, Angela, Zhong, Xiaotian, McKenna, Matthew, Francis, Christopher, Olland, Stephane, Bloom, Laird, Paulsen, Janet, Somers, Will, Jensen, Allan, Lin, Laura, Finlay, William JJ, Cunningham, Orla
Published in mAbs (01.11.2013)
Published in mAbs (01.11.2013)
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Journal Article
캐핑된 및 캐핑되지 않은 항체 시스테인, 및 항체-약물 접합에서 이들의 용도
PRASHAD AMARNAUTH SHASTRIE, LETENDRE LEO JOSEPH, HE TAO, SOMERS WILL, WANG WENGE, ZHONG XIAOTIAN, KRIZ RONALD WILLIAM
Year of Publication 13.04.2018
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Year of Publication 13.04.2018
Patent
Automated systems for protein crystallization
Bard, Joel, Ercolani, Kimberly, Svenson, Kristine, Olland, Andrea, Somers, Will
Published in Methods (San Diego, Calif.) (01.11.2004)
Published in Methods (San Diego, Calif.) (01.11.2004)
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Journal Article
Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39
Zhong, Xiaotian, Buddha, Madhavan, Guidotti, Guido, Kriz, Ron, Somers, Will, Mosyak, Lidia
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01.11.2008)
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01.11.2008)
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Journal Article
Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation
WENGE WANG, Leo Joseph LETENDRE, Amarnauth Shastrie PRASHAD, Will SOMERS, Ronald William Kriz, Xiaotian Zhong, Tao HE
Year of Publication 01.08.2022
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Year of Publication 01.08.2022
Patent