Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu
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Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain : resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe
MELLOR, G. W, THOMAS, E. W, TOPHAM, C. M, BROCKLEHURST, K
Published in Biochemical journal (15.02.1993)
Published in Biochemical journal (15.02.1993)
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Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors : the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain
THOMAS, M. P, TOPHAM, C. M, KOWLESSUR, D, MELLOR, G. W, THOMAS, E. W, WHITFORD, D, BROCKLEHURST, K
Published in Biochemical journal (15.06.1994)
Published in Biochemical journal (15.06.1994)
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A polyclonal antibody preparation with Michaelian catalytic properties
GALLACHER, G, JACKSON, C. S, SEARCEY, M, BADMAN, G. T, GOEL, R, TOPHAM, C. M, MELLOR, G. W, BROCKLEHURST, K
Published in Biochemical journal (01.11.1991)
Published in Biochemical journal (01.11.1991)
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Variation in the P2-S2 stereochemical selectivity towards the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin
PATEL, M, KAYANI, I. S, MELLOR, G. W, SREEDHARAN, S, TEMPLETON, W, THOMAS, E. W, THOMAS, M, BROCKLEHURST, K
Published in Biochemical journal (15.01.1992)
Published in Biochemical journal (15.01.1992)
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Site Distribution in Resin Beads as Determined by Confocal Raman Spectroscopy
Kress, Jürgen, Rose, Abigail, Frey, Jeremy G., Brocklesby, William S., Ladlow, Mark, Mellor, Geoff W., Bradley, Mark
Published in Chemistry : a European journal (17.09.2001)
Published in Chemistry : a European journal (17.09.2001)
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Development and validation of a fluoroimmunoassay for urinary normetanephrine
Gallacher, G, Mellor, G W, Landon, J, Sjostrom, L
Published in Annals of clinical biochemistry (01.09.1991)
Published in Annals of clinical biochemistry (01.09.1991)
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Catalytic antibody activity elicited by active immunisation : evidence for natural variation involving preferential stabilization of the transition state
GALLACHER, G, JACKON, C. S, SEARCEY, M, GOEL, R, MELLOR, G. W, SMITH, C. Z, BROCKLEHURST, K
Published in European journal of biochemistry (15.05.1993)
Published in European journal of biochemistry (15.05.1993)
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Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group
Mellor, G W, Sreedharan, S K, Kowlessur, D, Thomas, E W, Brocklehurst, K
Published in Biochemical journal (15.02.1993)
Published in Biochemical journal (15.02.1993)
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Characterization of the Electrostatic Perturbation of a Catalytic Site (Cys)-S –/(His)-Im +H Ion-pair in One Type of Serine Proteinase Architecture by Kinetic and Computational Studies on Chemically Mutated Subtilisin Variants
Plou, Francisco J., Kowlessur, Devanand, Malthouse, Paul G.J., Mellor, Geoffrey W., Hartshorn, Michael J., Pinitglang, Surapong, Patel, Hasu, Topham, Christopher M., Thomas, Emrys W., Verma, Chandra, Brocklehurst, Keith
Published in Journal of molecular biology (19.04.1996)
Published in Journal of molecular biology (19.04.1996)
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The kinetically influential ionizations of chymopapain M
Thomas, M P, Mellor, G W, Brocklehurst, K
Published in Biochemical Society transactions (01.05.1993)
Published in Biochemical Society transactions (01.05.1993)
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Ficin: a cysteine proteinase with binding site-catalytic site signalling characteristics intermediate between those of papain and actinidin
Patel, M, Thomas, M P, Kayani, I S, Mellor, G W, Thomas, E W, Brocklehurst, K
Published in Biochemical Society transactions (01.05.1993)
Published in Biochemical Society transactions (01.05.1993)
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New substrate-derived thiol-specific time-dependent inhibitors for the characterisation of the coupling of binding site interactions with catalytic site chemistry in the cysteine proteinases
Mellor, G W, Patel, M, Thomas, M P, Kowlessur, D, Sreedharan, S K, Thomas, E W, Brocklehurst, K
Published in Biochemical Society transactions (01.05.1993)
Published in Biochemical Society transactions (01.05.1993)
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Attenuation of Murine Collagen-Induced Arthritis by a Novel, Potent, Selective Small Molecule Inhibitor of IκB Kinase 2, TPCA-1 (2-[(Aminocarbonyl)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide), Occurs via Reduction of Proinflammatory Cytokines and Antigen-Induced T Cell Proliferation
Podolin, Patricia L., Callahan, James F., Bolognese, Brian J., Li, Yue H., Carlson, Karey, Davis, T. Gregg, Mellor, Geoff W., Evans, Christopher, Roshak, Amy K.
Published in The Journal of pharmacology and experimental therapeutics (01.01.2005)
Published in The Journal of pharmacology and experimental therapeutics (01.01.2005)
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Journal Article
Catalytic-site characteristics of the porcine calpain II 80 kDa/18kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors : evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group
MELLOR, G. W, SREEDHARAN, S. K, KOWLESSUR, D, THOMAS, E. W, BROCKLEHURST, K
Published in Biochemical journal (1993)
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Published in Biochemical journal (1993)
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