A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells
Vakulskas, Christopher A, Dever, Daniel P, Rettig, Garrett R, Turk, Rolf, Jacobi, Ashley M, Collingwood, Michael A, Bode, Nicole M, McNeill, Matthew S, Yan, Shuqi, Camarena, Joab, Lee, Ciaran M, Park, So Hyun, Wiebking, Volker, Bak, Rasmus O, Gomez-Ospina, Natalia, Pavel-Dinu, Mara, Sun, Wenchao, Bao, Gang, Porteus, Matthew H, Behlke, Mark A
Published in Nature medicine (01.08.2018)
Published in Nature medicine (01.08.2018)
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Journal Article
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins
Quadros, Rolen M, Miura, Hiromi, Harms, Donald W, Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P, Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M, Seshacharyulu, Parthasarathy, Batra, Surinder K, Behlke, Mark A, Zeiner, Sarah A, Jacobi, Ashley M, Izu, Yayoi, Thoreson, Wallace B, Urness, Lisa D, Mansour, Suzanne L, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B
Published in Genome Biology (17.05.2017)
Published in Genome Biology (17.05.2017)
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Journal Article
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Rosenblum, Daniel, Gutkin, Anna, Kedmi, Ranit, Ramishetti, Srinivas, Veiga, Nuphar, Jacobi, Ashley M, Schubert, Mollie S, Friedmann-Morvinski, Dinorah, Cohen, Zvi R, Behlke, Mark A, Lieberman, Judy, Peer, Dan
Published in Science advances (01.11.2020)
Published in Science advances (01.11.2020)
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Journal Article
A versatile, high-efficiency platform for CRISPR-based gene activation
Heidersbach, Amy J, Dorighi, Kristel M, Gomez, Javier A, Jacobi, Ashley M, Haley, Benjamin
Published in Nature communications (17.02.2023)
Published in Nature communications (17.02.2023)
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Journal Article
Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes
Kow, Rebecca L., Strovas, Timothy J., McMillan, Pamela J., Jacobi, Ashley M., Behlke, Mark A., Saxton, Aleen D., Latimer, Caitlin S., Keene, C. Dirk, Kraemer, Brian C.
Published in Neurobiology of disease (01.01.2021)
Published in Neurobiology of disease (01.01.2021)
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Journal Article
microRNA network regulates expression and biosynthesis of wild-type and ΔF508 mutant cystic fibrosis transmembrane conductance regulator
Ramachandran, Shyam, Karp, Philip H, Jiang, Peng, Ostedgaard, Lynda S, Walz, Amy E, Fisher, John T, Keshavjee, Shaf, Lennox, Kim A, Jacobi, Ashley M, Rose, Scott D, Behlke, Mark A, Welsh, Michael J, Xing, Yi, McCray, Paul B
Published in Proceedings of the National Academy of Sciences - PNAS (14.08.2012)
Published in Proceedings of the National Academy of Sciences - PNAS (14.08.2012)
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Journal Article
Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System
Shapiro, Jenny, Iancu, Ortal, Jacobi, Ashley M., McNeill, Matthew S., Turk, Rolf, Rettig, Garrett R., Amit, Ido, Tovin-Recht, Adi, Yakhini, Zohar, Behlke, Mark A., Hendel, Ayal
Published in Molecular therapy. Methods & clinical development (12.06.2020)
Published in Molecular therapy. Methods & clinical development (12.06.2020)
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Journal Article
Pharmacological interventions enhance virus-free generation of TRAC-replaced CAR T cells
Kath, Jonas, Du, Weijie, Pruene, Alina, Braun, Tobias, Thommandru, Bernice, Turk, Rolf, Sturgeon, Morgan L., Kurgan, Gavin L., Amini, Leila, Stein, Maik, Zittel, Tatiana, Martini, Stefania, Ostendorf, Lennard, Wilhelm, Andreas, Akyüz, Levent, Rehm, Armin, Höpken, Uta E., Pruß, Axel, Künkele, Annette, Jacobi, Ashley M., Volk, Hans-Dieter, Schmueck-Henneresse, Michael, Stripecke, Renata, Reinke, Petra, Wagner, Dimitrios L.
Published in Molecular therapy. Methods & clinical development (09.06.2022)
Published in Molecular therapy. Methods & clinical development (09.06.2022)
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Journal Article
An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
Dewari, Pooran Singh, Southgate, Benjamin, Mccarten, Katrina, Monogarov, German, O'Duibhir, Eoghan, Quinn, Niall, Tyrer, Ashley, Leitner, Marie-Christin, Plumb, Colin, Kalantzaki, Maria, Blin, Carla, Finch, Rebecca, Bressan, Raul Bardini, Morrison, Gillian, Jacobi, Ashley M, Behlke, Mark A, von Kriegsheim, Alex, Tomlinson, Simon, Krijgsveld, Jeroen, Pollard, Steven M
Published in eLife (11.04.2018)
Published in eLife (11.04.2018)
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Journal Article
Efficient delivery of RNA interference oligonucleotides to polarized airway epithelia in vitro
Ramachandran, Shyam, Krishnamurthy, Sateesh, Jacobi, Ashley M, Wohlford-Lenane, Christine, Behlke, Mark A, Davidson, Beverly L, McCray, Jr, Paul B
Published in American journal of physiology. Lung cellular and molecular physiology (01.07.2013)
Published in American journal of physiology. Lung cellular and molecular physiology (01.07.2013)
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Journal Article
CRISPAltRations: A validated cloud-based approach for interrogation of double-strand break repair mediated by CRISPR genome editing
Kurgan, Gavin, Turk, Rolf, Li, Heng, Roberts, Nathan, Rettig, Garrett R., Jacobi, Ashley M., Tso, Lauren, Sturgeon, Morgan, Mertens, Massimo, Noten, Roel, Florus, Kurt, Behlke, Mark A., Wang, Yu, McNeill, Matthew S.
Published in Molecular therapy. Methods & clinical development (11.06.2021)
Published in Molecular therapy. Methods & clinical development (11.06.2021)
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Journal Article
RNA inhibition highlights cyclin D1 as a potential therapeutic target for mantle cell lymphoma
Weinstein, Shiri, Emmanuel, Rafi, Jacobi, Ashley M, Abraham, Avigdor, Behlke, Mark A, Sprague, Andrew G, Novobrantseva, Tatiana I, Nagler, Arnon, Peer, Dan
Published in PloS one (14.08.2012)
Published in PloS one (14.08.2012)
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Journal Article
Inosine-Mediated Modulation of RNA Sensing by Toll-Like Receptor 7 (TLR7) and TLR8
Sarvestani, Soroush T, Tate, Michelle D, Moffat, Jessica M, Jacobi, Ashley M, Behlke, Mark A, Miller, Alistair R, Beckham, Simone A, McCoy, Claire E, Chen, Weisan, Mintern, Justine D, O'Keeffe, Meredith, John, Matthias, Williams, Bryan R G, Gantier, Michael P
Published in Journal of Virology (01.01.2014)
Published in Journal of Virology (01.01.2014)
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Journal Article
Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish
Hoshijima, Kazuyuki, Jurynec, Michael J., Klatt Shaw, Dana, Jacobi, Ashley M., Behlke, Mark A., Grunwald, David Jonah
Published in Developmental cell (02.12.2019)
Published in Developmental cell (02.12.2019)
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Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development
Bégin-Lavallée, Valérie, Midavaine, Élora, Dansereau, Marc-André, Tétreault, Pascal, Longpré, Jean-Michel, Jacobi, Ashley M, Rose, Scott D, Behlke, Mark A, Beaudet, Nicolas, Sarret, Philippe
Published in Molecular pain (2016)
Published in Molecular pain (2016)
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Journal Article
Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes
Jacobi, Ashley M., Rettig, Garrett R., Turk, Rolf, Collingwood, Michael A., Zeiner, Sarah A., Quadros, Rolen M., Harms, Donald W., Bonthuis, Paul J., Gregg, Christopher, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B., Behlke, Mark A.
Published in Methods (San Diego, Calif.) (15.05.2017)
Published in Methods (San Diego, Calif.) (15.05.2017)
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Journal Article
Post-transcriptional regulation of cystic fibrosis transmembrane conductance regulator expression and function by microRNAs
Ramachandran, Shyam, Karp, Philip H, Osterhaus, Samantha R, Jiang, Peng, Wohlford-Lenane, Christine, Lennox, Kim A, Jacobi, Ashley M, Praekh, Kal, Rose, Scott D, Behlke, Mark A, Xing, Yi, Welsh, Michael J, McCray, Jr, Paul B
Published in American journal of respiratory cell and molecular biology (01.10.2013)
Published in American journal of respiratory cell and molecular biology (01.10.2013)
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Journal Article
Correlating In Vitro Splice Switching Activity With Systemic In Vivo Delivery Using Novel ZEN-modified Oligonucleotides
Hammond, Suzan M, McClorey, Graham, Nordin, Joel Z, Godfrey, Caroline, Stenler, Sofia, Lennox, Kim A, Smith, CI Edvard, Jacobi, Ashley M, Varela, Miguel A, Lee, Yi, Behlke, Mark A, Wood, Matthew J A, Andaloussi, Samir E L
Published in Molecular therapy. Nucleic acids (2014)
Published in Molecular therapy. Nucleic acids (2014)
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