Search Results - "Han Teng Wong"

Developing Gram-negative bacteria for the secretion of heterologous proteins by Burdette, Lisa Ann, Leach, Samuel Alexander, Wong, Han Teng, Tullman-Ercek, Danielle

“…Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in…”

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An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system by Burdette, Lisa Ann, Wong, Han Teng, Tullman-Ercek, Danielle

“…Protein secretion in bacteria is an attractive strategy for heterologous protein production because it retains the high titers and tractability of bacterial…”

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A sweeter future: Using protein language models for exploring sweeter brazzein homologs by Nicholas Chua, Bryan, Mei Guo, Wei, Teng Wong, Han, Siak-Wei Ow, Dave, Leng Ho, Pooi, Koh, Winston, Koay, Ann, Tian Wong, Fong

“…•Protein language models were used to design new brazzein homologs.•Unexpected beneficial mutations were uncovered with this approach.•A more heat-resistant…”

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BAsE-Seq: a method for obtaining long viral haplotypes from short sequence reads by Hong, Lewis Z, Hong, Shuzhen, Wong, Han Teng, Aw, Pauline PK, Cheng, Yan, Wilm, Andreas, de Sessions, Paola F, Lim, Seng Gee, Nagarajan, Niranjan, Hibberd, Martin L, Quake, Stephen R, Burkholder, William F

“…We present a method for obtaining long haplotypes, of over 3 kb in length, using a short-read sequencer, Barcode-directed Assembly for Extra-long Sequences…”

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Construction of a constitutively active type III secretion system for heterologous protein secretion by Liang, Julie Ming, Burdette, Lisa Ann, Wong, Han Teng, Tullman-Ercek, Danielle

“…Proteins comprise a multibillion-dollar industry in enzymes and therapeutics, but bacterial protein production can be costly and inefficient. Proteins of…”

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A Secretion-Amplification Role for Salmonella enterica Translocon Protein SipD by Glasgow, Anum Azam, Wong, Han Teng, Tullman-Ercek, Danielle

“…The bacterial type III secretion system (T3SS) is an important target for enabling high-titer production of proteins of biotechnological interest as well as…”

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BAsE-Seq: a method for obtaining long viral haplotypes from short sequence reads by Hong, Lewis Z, Hong, Shuzhen, Wong, Han Teng, Aw, Pauline P K, Cheng, Yan, Wilm, Andreas, de Sessions, Paola F, Lim, Seng Gee, Nagarajan, Niranjan, Hibberd, Martin L, Quake, Stephen R, Burkholder, William F

“…We present a method for obtaining long haplotypes, of over 3 kb in length, using a short-read sequencer, Barcode-directed Assembly for Extra-long Sequences…”

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BRAZZEIN VARIANT by CHUA, Bryan Nicholas, KOAY, Ann Zhufang, GUO, Weimei, WONG, Fong Tian, WONG, Han Teng

“…There is provided a brazzein variant comprising a circular permutation brazzein. Also disclosed are polynucleotides, vector, host cells, and methods of…”
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RECOMBINANT STRAINS AND MEDIUM FORMULATION FOR ENHANCING SECRETION TITER USING A TYPE III SECRETION SYSTEM by BURDETTE, Lisa, A, WONG, Han, Teng, ERCEK, Danielle Tullman

“…The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression…”
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RECOMBINANT STRAINS AND MEDIUM FORMULATION FOR ENHANCING SECRETION TITER USING A TYPE III SECRETION SYSTEM by Ercek, Danielle Tullman, Burdette, Lisa A, Wong, Han Teng

“…The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression…”
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BRAZZEIN VARIANTS by CHUA, Bryan Nicholas, KOAY, Ann Zhufang, GUO, Weimei, KOH, Lian Chye Winston, WONG, Fong Tian, WONG, Han Teng

“…There is provided a brazzein variant comprising X1X2KCKX6X7YX9NYPX13X14KCX17X18AX20QCNYDCKLDKHARX34GECFYDEX42RNX45 X46CX48CDYCX53YX55 (SEQ ID NO: 1), wherein…”
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RECOMBINANT STRAINS AND MEDIUM FORMULATION FOR ENHANCING SECRETION TITER USING A TYPE III SECRETION SYSTEM by BURDETTE, Lisa, A, WONG, Han, Teng, ERCEK, Danielle Tullman

“…The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression…”
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An Optimized Growth Medium for Increased Recombinant Protein Secretion Titer via the Type III Secretion System by Burdette, Lisa, Han Teng Wong, Tullman-Ercek, Danielle

“…Background Protein secretion in bacteria is an attractive strategy for heterologous protein production because it retains the high titers and tractability of…”

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Construction of a constitutively active type III secretion system for heterologous protein secretion by Liang, Julie Ming, Burdette, Lisa Ann, Wong, Han Teng, Tullman-Ercek, Danielle

“…Proteins comprise a multibillion-dollar industry in enzymes and therapeutics, but bacterial protein production can be costly and inefficient. Proteins of…”

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A sweeter future: Using protein language models for exploring sweeter brazzein homologs by Chua, Bryan Nicholas, Guo, Wei Mei, Wong, Han Teng, Ow, Dave Siak-Wei, Ho, Pooi Leng, Koh, Winston, Koay, Ann, Wong, Fong Tian

“…Reducing sugar intake lowers the risk of obesity and associated metabolic disorders. Currently, this is achieved using artificial non-nutritive sweeteners,…”

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