Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms
Claassens, Nico J, Siliakus, Melvin F, Spaans, Sebastiaan K, Creutzburg, Sjoerd C A, Nijsse, Bart, Schaap, Peter J, Quax, Tessa E F, van der Oost, John
Published in PloS one (2017)
Published in PloS one (2017)
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The miniature CRISPR-Cas12m effector binds DNA to block transcription
Wu, Wen Y., Mohanraju, Prarthana, Liao, Chunyu, Adiego-Pérez, Belén, Creutzburg, Sjoerd C.A., Makarova, Kira S., Keessen, Karlijn, Lindeboom, Timon A., Khan, Tahseen S., Prinsen, Stijn, Joosten, Rob, Yan, Winston X., Migur, Anzhela, Laffeber, Charlie, Scott, David A., Lebbink, Joyce H.G., Koonin, Eugene V., Beisel, Chase L., van der Oost, John
Published in Molecular cell (01.12.2022)
Published in Molecular cell (01.12.2022)
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Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a
Creutzburg, Sjoerd C.A., Swartjes, Thomas, van der Oost, John
Published in Methods (San Diego, Calif.) (01.02.2020)
Published in Methods (San Diego, Calif.) (01.02.2020)
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Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas
Patinios, Constantinos, Creutzburg, Sjoerd C A, Arifah, Adini Q, Adiego-Pérez, Belén, Gyimah, Evans A, Ingham, Colin J, Kengen, Servé W M, van der Oost, John, Staals, Raymond H J
Published in Nucleic acids research (08.11.2021)
Published in Nucleic acids research (08.11.2021)
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Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a
Creutzburg, Sjoerd C A, Wu, Wen Y, Mohanraju, Prarthana, Swartjes, Thomas, Alkan, Ferhat, Gorodkin, Jan, Staals, Raymond H J, van der Oost, John
Published in Nucleic acids research (06.04.2020)
Published in Nucleic acids research (06.04.2020)
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