Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
Ayoubi, Riham, Ryan, Joel, Biddle, Michael S, Alshafie, Walaa, Fotouhi, Maryam, Bolivar, Sara Gonzalez, Ruiz Moleon, Vera, Eckmann, Peter, Worrall, Donovan, McDowell, Ian, Southern, Kathleen, Reintsch, Wolfgang, Durcan, Thomas M, Brown, Claire, Bandrowski, Anita, Virk, Harvinder, Edwards, Aled M, McPherson, Peter, Laflamme, Carl
Published in eLife (23.11.2023)
Published in eLife (23.11.2023)
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Journal Article
Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence [version 3; peer review: 1 approved, 2 approved with reservations]
Alshafie, Walaa, Ayoubi, Riham, Fotouhi, Maryam, Southern, Kathleen, Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
Identification of high-performing antibodies for SPARC-related modular calcium-binding protein 1 (SMOC-1) for use in Western Blot and immunoprecipitation [version 2; peer review: 1 approved, 1 approved with reservations]
Ayoubi, Riham, González Bolívar, Sara, Nicouleau, Michael, Southern, Kathleen, Laflamme, Carl
Published in F1000 research (01.01.2023)
Published in F1000 research (01.01.2023)
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Journal Article
A guide to selecting high-performing antibodies for amyloid-beta precursor protein for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 3 approved, 1 approved with reservations]
Ayoubi, Riham, Fotouhi, Maryam, Worrall, Donovan, Southern, Kathleen, Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 1 approved]
Alshafie, Walaa, Fotouhi, Maryam, Ayoubi, Riham, Southern, Kathleen, Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 2 approved]
Worrall, Donovan, Ayoubi, Riham, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence [version 2; peer review: 3 approved with reservations]
Ayoubi, Riham, Fotouhi, Maryam, Alende, Charles, Ruíz Moleón, Vera, Southern, Kathleen, Laflamme, Carl
Published in F1000 research (01.01.2024)
Published in F1000 research (01.01.2024)
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Journal Article
The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 2 approved]
McDowell, Ian, Ayoubi, Riham, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence [version 1; peer review: 1 approved, 2 approved with reservations]
Ayoubi, Riham, McDowell, Ian, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence [version 1; peer review: 2 approved]
Alshafie, Walaa, Fotouhi, Maryam, Ayoubi, Riham, Shlaifer, Irina, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence [version 2; peer review: 1 approved, 1 approved with reservations]
Ayoubi, Riham, Alshafie, Walaa, Shlaifer, Irina, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (01.01.2023)
Published in F1000 research (01.01.2023)
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Journal Article
A guide to selecting high-performing antibodies for RNA-binding protein TIA1 for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 1 approved, 1 approved with reservations]
Fotouhi, Maryam, Worrall, Donovan, Ayoubi, Riham, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence
Ruíz Moleón, Vera, Alende, Charles, Fotouhi, Maryam, Southern, Kathleen, Ayoubi, Riham, Laflamme, Carl
Published in F1000 research (01.09.2024)
Published in F1000 research (01.09.2024)
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Journal Article
The identification of high-performing antibodies for Coiled-coil-helix-coiled-coil-helix domain containing protein 10 (CHCHD10) for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 2 approved, 1 approved with reservations]
Ayoubi, Riham, Alshafie, Walaa, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Published in F1000 research (2023)
Published in F1000 research (2023)
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Journal Article
A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry [version 1; peer review: awaiting peer review]
Biddle, Michael S., Alende, Charles, Fotouhi, Maryam, Jones, Carolyn, Ayoubi, Riham, Southern, Kathleen, Laflamme, Carl, Virk, Harvinder
Published in F1000 research (2024)
Published in F1000 research (2024)
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Journal Article