The white confocal : microscopic optical sectioning in all colors

This book offers a comprehensive introduction to confocal microscopy - with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an o...

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Bibliographic Details
Main Author Borlinghaus, Rolf Theodor
Format Electronic eBook
LanguageEnglish
Published Cham : Springer, 2017.
Subjects
Confocal microscopy.
elektronické knihy
electronic books
Online AccessPlný text

Cover

Table of Contents:
  • Preface; Contents; About the Author; 1 Microscopy-Introduction; 1.1 Lenses; 1.2 The Microscope; 1.2.1 The Objective Lens; 1.2.2 The Eyepiece; 1.2.3 The Compound Microscope; 1.3 Resolution and Resolution Limits; 1.3.1 Abbe's Formula; 1.3.2 Luminous Dots; 1.3.3 The Full Width Half Maximum; 1.3.4 So, What Is the Correct Answer?; 1.4 Beyond the Resolution Limit; Further Reading; 2 Fluorescence; 2.1 What Is Fluorescence?; 2.1.1 The Fluorescence Process; 2.1.2 Color Games; 2.1.3 Lifetimes; 2.2 Fluorescence and Microscopy; 2.2.1 Power Ratio of Excitation and Emission.
  • 2.2.2 Transmitted Light Fluorescence and Incident Light Fluorescence2.2.3 Illumination; 2.2.4 Excitation Filter; 2.2.5 Incident Light Beam Splitters; 2.2.6 Emission Filter; 2.3 Artificial Colors; Further Reading; 3 Confocal Microscopy; 3.1 The Motive; 3.2 The Principle; 3.2.1 Spot Illumination; 3.2.2 Point Detection; 3.3 The Scanned Image; 3.3.1 Recording; 3.3.2 Scan Procedure; 3.3.3 Slice Thickness; 3.3.4 The Third Dimension; 3.4 Two-Photon Fluorescence Microscopy; 3.5 Elements of a Confocal Microscope; 3.5.1 Light Source; 3.5.2 Excitation Filter; 3.5.3 Primary Beam Splitter.
  • 3.5.4 Beam Scanner3.5.5 Objective Lens; 3.5.6 Channel Separation; 3.5.7 Emission Filter; 3.5.8 Sensors; 3.6 The "White Confocal"; Further Reading; 4 Light Sources; 4.1 Laser; 4.2 Laser Types; 4.3 White Light Laser; Further Reading; 5 Acousto-Optical Excitation Filter; 5.1 How Does an Acousto-Optical Filter Work?; 5.2 Multichannel Dimmer for Laser Light; 5.3 Spectral Freedom: AOTF and White Light Laser; Further Reading; 6 White Beam Splitting; 6.1 Acousto-Optical Beam Splitter; 6.2 AOBS and White Light Source; Further Reading; 7 Splitting of Emissions; 7.1 Prism; 7.1.1 Optical Grating.
  • 7.2 Continuous Change of Direction: The SpectrumFurther Reading; 8 Emission Filtering; 8.1 The Line Detector; 8.2 The Multiband Detector; Further Reading; 9 Separation in the Time Domain; 9.1 Sensors Sense Photons; 9.1.1 Photomultiplier Tubes; 9.1.2 Avalanche Photodiodes; 9.1.3 Hybrid Detector; 9.2 White Fluorescence Measurement: FLIM; 9.3 A White Filter with High Selectivity; Further Reading.

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