Single cell analysis : contemporary research and clinical applications

This book highlights the current state of the art in single cell analysis, an area that involves many fields of science - from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings togethe...

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Bibliographic Details
Other Authors Robinson, J. Paul (Editor), Cossarizza, Andrea, 1958- (Editor)
Format Electronic eBook
LanguageEnglish
Published Singapore : Springer, 2017.
SeriesSeries in bioengineering.
Subjects
Flow cytometry.
elektronické knihy
electronic books
Online AccessPlný text

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Table of Contents:
  • Preface; Acknowledgements; Contents; Contributors; 1 Microenvironment Cytometry; Abstract; 1 Microcommunities and Microenvironments; 1.1 Introduction; 1.1.1 Boundaries and Niches; 1.1.2 Tumor Microenvironment [TME]; 2 Plasticity of Cellular Communities; 2.1 Landscapes and Attractor States; 2.2 Cell Transitions and Interactions: The Glycome; 2.3 Cellular Interactions in a Microcommunity: Transitions in NCAM Polysialylation; 3 Chromosomal Instability (CIN) and the Microenvironment; 3.1 Cytometric Analysis of Chromosomal Instability; 3.2 Mitotic Bypass; 3.3 Mitotic Bypass and the p53 Network.
  • 4 In Vitro Microenvironment Models and Hollow Fiber Technology4.1 3D Models: Multicellular Tumor Spheroids; 4.2 Encapsulation Systems; 4.2.1 Microencapsulation and Macroencapsulation; 4.2.2 HF Bioreactor (HFB) Cell-Culture Systems; 4.2.3 HF Microenvironment Control; 4.2.4 HF Implant Assay; 4.2.5 HFA Cytometry; 5 Microenvironment and Hypoxia; 5.1 Modeling the Hypoxic Microenvironment; 5.2 Stromal-Tumor Hypoxia; 5.3 Hypoxia Detection; 5.4 Cellular Responses to the Hypoxic Tumor Environment; 6 Targeting Hypoxia; 6.1 The Therapeutic Paradigm; 6.2 Hypoxia-Activated Prodrugs: HAPs.
  • 6.3 Unidirectional HAPs (uHAPs) and Multilevel Targeting7 The Continuing Challenge; Acknowledgements and Declarations; References; 2 Rare Cells: Focus on Detection and Clinical Relevance; Abstract; 1 Introduction; 2 Ag-Specific T Cells; 3 Invariant Natural Killer T (iNKT) Cells; 4 Circulating Endothelial Cells and Their Precursors; 5 Circulating Tumor Cells; 6 Analysis of Rare Events by Flow Cytometry: The First Step; 6.1 Enrichment and Choice of Markers; 6.2 Number of Acquired Events; 6.3 Sample Concentration and Flow Rate; 6.4 Thresholds, Gating, and DUMP Channel; 6.5 Data Analysis.
  • 4 Cytomics of Oxidative Stress: Probes and ProblemsAbstract; 1 Introduction to Reactive Oxygen (ROS) and Nitrogen (RNS) Species; 2 The Physiological Side of ROS and NOS; 2.1 Sources of ROS and RNS; 2.2 ROS in Phagocytosis; 2.3 ROS and RNS in Cellular Signaling; 3 Oxidative Stress: Definition, Causes, and Consequences; 3.1 Definition of Oxidative Stress; 3.2 Causes of Oxidative Stress; 3.3 Consequences of Oxidative Stress; 3.3.1 Protein Damage; 3.3.2 Lipid Peroxidation; 3.3.3 Oxidative Lesions to DNA; 4 Strategies and Reagents for Cytomic Analysis of Oxidative Stress.

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